STEREOSPECIFIC HYDROXYLATION OF INDAN BY ESCHERICHIA-COLI CONTAINING THE CLONED TOLUENE DIOXYGENASE GENES FROM PSEUDOMONAS-PUTIDA F1

被引：39
|
作者：
BRAND, JM
CRUDEN, DL
ZYLSTRA, GJ
GIBSON, DT
机构：
[1] UNIV IOWA,DEPT MICROBIOL,IOWA CITY,IA 52242
[2] UNIV IOWA,CTR BIOCATALYSIS & BIOPROC,IOWA CITY,IA 52242
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1992年 / 58卷 / 10期
关键词：
D O I：
10.1128/AEM.58.10.3407-3409.1992
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Escherichia coli JM109(pDTG601), containing the todC1C2BA genes encoding toluene dioxygenase from Pseudomonas putida F1, oxidizes indan to (-)-(1R)-indanol (83% R) and trans-1,3-indandiol. Under similar conditions, P. putida F39/D oxidizes indan to (-)-(1R)-indanol (96% R), 1-indanone, and trans-1,3-indandiol. The differences in the enantiomeric composition of the 1-indanols formed by the two organisms are due to the presence of a 1-indanol dehydrogenase in P. putida F39/D that preferentially oxidizes (+)-(1S)-indanol.
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页码：3407 / 3409
页数：3
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