HIGH-LEVEL EXPRESSION OF FUSARIUM ALKALINE PROTEASE GENE IN ACREMONIUM-CHRYSOGENUM

被引：17

作者：

MORITA, S ^{[1
]}

KURIYAMA, M ^{[1
]}

NAKATSU, M ^{[1
]}

KITANO, K ^{[1
]}

机构：

[1] TAKEDA CHEM IND LTD, PROD RES LABS, DIV PHARMACEUT DEV, YODOGAWA KU, OSAKA 532, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1994年 / 58卷 / 04期

关键词：

D O I：

10.1271/bbb.58.627

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We transformed Acremonium chrysogenum with the genomic DNA of the alkaline protease (Alp) from Fusarium sp. S-19-5 including its promoter. Most of the transformants thus obtained produced a large amount of Alp. PCR and Southern hybridization analysis of genomic DNAs from these transformants showed chromosomal integration of the full-length Alp gene. SDS-PAGE analysis of the supernatant from the transformants showed the presence of Fusarium Alp. The amino terminus of the Alp produced in A. chrysogenum was identical to that of native Fusarium Alp. These results indicate that the Alp promoter, signal sequence, and introns functioned correctly in A. chrysogenum. One of the transformants produced more than 4g of the Alp per liter in a jar fermenter.

引用

页码：627 / 630

页数：4

共 50 条

[21] CONTROLLED HIGH-LEVEL EXPRESSION OF THE LON GENE OF ESCHERICHIA-COLI ALLOWS OVERPRODUCTION OF LON PROTEASE
THOMAS, CD
MODHA, J
RAZZAQ, TM
CULLIS, PM
RIVETT, AJ
GENE, 1993, 136 (1-2) : 237 - 242
[22] Expression of the Acremonium chrysogenumcefT gene in Penicillum chrysogenum indicates that it encodes an hydrophilic β-lactam transporter
Ricardo V. Ullán
Fernando Teijeira
Juan F. Martín
Current Genetics, 2008, 54 : 153 - 161
[23] A mammalianized synthetic nitroreductase gene for high-level expression
Grohmann, Maik
Paulmann, Nils
Fleischhauer, Sebastian
Vowinckel, Jakob
Priller, Josef
Walther, Diego J.
BMC CANCER, 2009, 9
[24] A mammalianized synthetic nitroreductase gene for high-level expression
Maik Grohmann
Nils Paulmann
Sebastian Fleischhauer
Jakob Vowinckel
Josef Priller
Diego J Walther
BMC Cancer, 9
[25] Expression of a cephalosporin C esterase gene in Acremonium chrysogenum for the direct production of deacetylcephalosporin C
Basch, J
Franceschini, T
Tonzi, S
Chiang, SJD
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2004, 31 (11) : 531 - 539
[26] CLONING WITH TANDEM GENE SYSTEMS FOR HIGH-LEVEL GENE-EXPRESSION
LEE, N
COZZITORTO, J
WAINWRIGHT, N
TESTA, D
NUCLEIC ACIDS RESEARCH, 1984, 12 (17) : 6797 - 6812
[27] High-level expression of an alkaline lipase gene from acinetobacter radioresistens CMC-1 in Pichia pastoris
Lin, Y., 1600, South China University of Technology, Guangzhou, 510640, China (29):
[28] High-level expression of bovine β-lactoglobulin gene in transgenic mice
Hyttinen, JM
Korhonen, VP
Hiltunen, MO
Myöhänen, S
Jänne, J
JOURNAL OF BIOTECHNOLOGY, 1998, 61 (03) : 191 - 198
[29] A new method for gene synthesis and its high-level expression
Cao, Shi-shu
Hu, Zhi-qiu
JOURNAL OF MICROBIOLOGICAL METHODS, 2009, 79 (01) : 106 - 110
[30] The high-level similarity of some disparate gene expression measures
Raghavan, Nandini
De Bondt, An M. I. M.
Talloen, Willem
Moechars, Dieder
Gohlmann, Hinrich W. H.
Amaratunga, Dhammika
BIOINFORMATICS, 2007, 23 (22) : 3032 - 3038

← 1 2 3 4 5 →