PHOSPHORYLATION OF A BACTERIAL ACTIVATOR PROTEIN, OMPR, BY A PROTEIN-KINASE, ENVZ, STIMULATES THE TRANSCRIPTION OF THE OMPF AND OMPC GENES IN ESCHERICHIA-COLI

被引：83

作者：

AIBA, H ^{[1
]}

MIZUNO, T ^{[1
]}

机构：

[1] NAGOYA UNIV,SCH AGR,MICROBIOL LAB,CHIKUSA KU,NAGOYA,AICHI 464,JAPAN

来源：

FEBS LETTERS | 1990年 / 261卷 / 01期

关键词：

(E. coli outer membrane); Activator protein; EnvZ; Kinase; OmpR; Phosphorylation; Transcription activation;

D O I：

10.1016/0014-5793(90)80626-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The OmpR of Escherichia coli is a positive regulator specific for the ompF and ompC genes, which encode outer membrane proteins OmpF and OmpC, respectively. The EnvZ protein is a protein kinase which specifically phosphorylates the OmpR protein. In this study, the results of in vitro transcription experiments revealed that the phosphorylation of the OmpR protein by the EnvZ protein stimulates the transcription of both the ompF and ompC genes. © 1990.

引用

页码：19 / 22

页数：4

共 15 条

[1]

AIBA H, 1989, J BIOL CHEM, V264, P14090

[2]

AIBA H, 1989, J BIOL CHEM, V264, P8563

[3] PHOSPHORYLATION OF A BACTERIAL ACTIVATOR PROTEIN, OMPR, BY A PROTEIN-KINASE, ENVZ, RESULTS IN STIMULATION OF ITS DNA-BINDING ABILITY [J].

AIBA, H ;

NAKASAI, F ;

MIZUSHIMA, S ;

MIZUNO, T .

JOURNAL OF BIOCHEMISTRY, 1989, 106 (01) :5-7

[4] PHOSPHORYLATION OF OMPR BY THE OSMOSENSOR ENVZ MODULATES EXPRESSION OF THE OMPF AND OMPC GENES IN ESCHERICHIA-COLI [J].

FORST, S ;

DELGADO, J ;

INOUYE, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (16) :6052-6056

[5] ISOLATION AND CHARACTERIZATION OF DELTA-OMPB STRAINS OF ESCHERICHIA-COLI BY A GENERAL-METHOD BASED ON GENE FUSIONS [J].

GARRETT, S ;

TAYLOR, RK ;

SILHAVY, TJ ;

BERMAN, ML .

JOURNAL OF BACTERIOLOGY, 1985, 162 (02) :840-844

[6] GENETIC-ANALYSIS OF THE MAJOR OUTER-MEMBRANE PROTEINS OF ESCHERICHIA-COLI [J].

HALL, MN ;

SILHAVY, TJ .

ANNUAL REVIEW OF GENETICS, 1981, 15 :91-142

[7] A BACTERIAL ENVIRONMENTAL SENSOR THAT FUNCTIONS AS A PROTEIN-KINASE AND STIMULATES TRANSCRIPTIONAL ACTIVATION [J].

IGO, MM ;

NINFA, AJ ;

SILHAVY, TJ .

GENES & DEVELOPMENT, 1989, 3 (05) :598-605

[8]

JO YL, 1986, J BIOL CHEM, V261, P5252

[9] DETERMINATION OF THE PROMOTER STRENGTH IN THE MIXED TRANSCRIPTION SYSTEM - PROMOTERS OF LACTOSE, TRYPTOPHAN AND RIBOSOMAL PROTEIN-L10 OPERON FROM ESCHERICHIA-COLI [J].

KAJITANI, M ;

ISHIHAMA, A .

NUCLEIC ACIDS RESEARCH, 1983, 11 (03) :671-686

[10] MOLECULAR ANALYSIS BY DELETION AND SITE-DIRECTED MUTAGENESIS OF THE CIS-ACTING UPSTREAM SEQUENCE INVOLVED IN ACTIVATION OF THE OMPF PROMOTER IN ESCHERICHIA-COLI [J].

KATO, M ;

AIBA, H ;

MIZUNO, T .

JOURNAL OF BIOCHEMISTRY, 1989, 105 (03) :341-347

← 1 2 →