DEGRADATION OF n-ALKANES IN OIL POLLUTED SOIL AFTER STIMULATION BY FYRE-ZYME ENZYME PREPARATION AND HYDROGEN PEROXIDE

Petroleum substances are widely used in many industries and are used as lubricants and fuels in motor vehicles. Due to the pipeline failure, lost of substances on the loading stations area and traffic crashes, petroleum contamination of soils are very common. High concentration of petroleum pollutants in the soil, leads to reduced activity of the indigenous microflora and extends their fate in the environment. However solubility in water of petroleum products is low, contamination could easily move from accident site to other places with surface and groundwater streams. It is especially important on water resources or agriculture areas. The high costs of removal of oil spills to the soil and ground, forces to search for low-cost and effective methods of soils decontamination by "in situ" methods. The aim of this study was to compare the effectiveness of biodegradation of diesel fuel and mineral oil in the soil contaminated with petroleum substances, after "Fyre-Zyme" enzyme reagent stimulation and/or hydrogen peroxide. Obtained results indicate on stimulation of degradation process of diesel fuel and mineral oil, either by used enzyme and hydrogen peroxide compared with the control samples in 60 days period of experiment. This indicates the possibility of use of tested additives for soils bioremediation processes as a cheap alternative for ex-situ methods.