SITE-SPECIFIC N-15-LABELING OF OLIGONUCLEOTIDES FOR NMR - THE TRP OPERATOR AND ITS INTERACTION WITH THE TRP REPRESSOR

被引：17
|
作者：
RAMESH, V
XU, YZ
ROBERTS, GCK
机构：
[1] UNIV LEICESTER, DEPT BIOCHEM, LEICESTER, LEICS, ENGLAND
[2] UNIV LEICESTER, BIOL NMR CTR, LEICESTER, LEICS, ENGLAND
[3] UCL, DEPT BIOCHEM & MOLEC BIOL, CANC RES CAMPAIGN, NITROSAMINE INDUCED CANC RES GRP, LONDON, ENGLAND
来源：
FEBS LETTERS | 1995年 / 363卷 / 1-2期
基金：
英国生物技术与生命科学研究理事会;
关键词：
NMR; STABLE ISOTOPE; PROTEIN-DNA INTERACTION;
D O I：
10.1016/0014-5793(95)00226-Y
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A convenient and economical method is described for the site-specific N-15-labelling of the 4-amino group of individual cytidine residues in oligonucleotides. This is applied to a 20 base-pair oligonucleotide corresponding to the trp operator; this oligonucleotide and its complex with the E. coli trp repressor are studied by NMR, using H-1-N-15 HMQC and N-15-edited H-1-H-1 NOESY experiments.
引用
收藏
页码：61 / 64
页数：4
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