CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF BACILLUS-STEAROTHERMOPHILUS FARNESYL DIPHOSPHATE SYNTHASE EXPRESSED IN ESCHERICHIA-COLI

被引：11

作者：

NAKANE, H

KOYAMA, T

OBATA, S

OSABE, M

TAKESHITA, A

NISHINO, T

OGURA, K

MIKI, K

机构：

[1] TOKYO INST TECHNOL,RESOURCES UTILIZAT RES LAB,MIDORI KU,YOKOHAMA,KANAGAWA 227,JAPAN

[2] TOYOTA MOTOR CO LTD,BIORES LAB,TOYOTA,AICHI 471,JAPAN

[3] TOHOKU UNIV,INST CHEM REACT SCI,AOBA KU,SENDAI,MIYAGI 980,JAPAN

[4] TOHOKU UNIV,FAC ENGN,DEPT BIOCHEM & ENGN,AOBA KU,SENDAI,MIYAGI 980,JAPAN

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 233卷 / 04期

关键词：

FARNESYL DIPHOSPHATE SYNTHASE; THERMOSTABLE ENZYME; PRENYLTRANSFERASE; CRYSTALLIZATION; X-RAY DIFFRACTION;

D O I：

10.1006/jmbi.1993.1554

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thermostable farnesyl diphosphate synthase (EC 2.5.1.10) from Bacillus stearothermophilus, which was overexpressed in Escherichia coli, has been crystallized by the vapor-diffusion procedure. Tetragonal crystals were obtained using ammonium sulfate as a precipitant. The crystals diffracted X-rays to about 3 Å resolution. The diffraction pattern indicated that the space group is I4122 with unit-cell dimensions of a = b = 114 Å and c = 247 Å. It is thought that the asymmetric unit comprises two or three molecules of farnesyl diphosphate synthase. © 1993 Academic Press Limited.

引用

页码：787 / 788

页数：2

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