PREPARATIVE LIQUID-CHROMATOGRAPHY .2. EXISTENCE OF OPTIMUM INJECTION CONDITIONS FOR OVERLOADED GRADIENT ELUTION SEPARATIONS

被引：5
|
作者：
CRETIER, G
ELKHABCHI, M
ROCCA, JL
机构：
[1] Laboratoire des Sciences Analytiques (UA CNRS 435), Université Claude Bernard Lyon I, 69622 Villeurbanne Cedex, Bat. 308, 43 Boulevard du
来源：
JOURNAL OF CHROMATOGRAPHY | 1992年 / 596卷 / 01期
关键词：
D O I：
10.1016/0021-9673(92)80198-4
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The effect of injection conditions on overloaded gradient elution separations in liquid chromatography was studied theoretically from simulated chromatograms. The simulation algorithm was based on the Craig machine. The separation of binary mixtures having both constant and non-constant separation factors with changing modulator concentration was examined. It appears that there is a general parallelism between gradient elution and isocratic elution under overload conditions: the injection concentration has to be optimized in order to maximize the recovered amount and the optimum injection conditions depend on the column efficiency.
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页码：15 / 25
页数：11
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