AN IMPROVED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ASSAY FOR ACETAZOLAMIDE

A high performance liquid chromatographic (HPLC) method for the quantitation of acetazolamide in a suspension dosage form is described. The method is simple, accurate, and precise. Acetazolamide was extracted from the suspension using a mobile phase which contained 65% phosphate buffer and 35% methanol and separated from other components by reversed-phase high performance liquid chromatography. The separation was carried out using a C18 column (250 x 4.5 mm i.d.). The photometric detector was set at 254 nm. The excipients present in the tablets from which the suspension was compounded did not interfere with the assay procedure. Sixteen replicate injections of 10.5 mg/mL samples resulted in a percent relative standard deviation of 2.02.