CATION-COUPLING IN CHIMERIC MELIBIOSE CARRIERS DERIVED FROM ESCHERICHIA-COLI AND KLEBSIELLA-PNEUMONIAE - THE AMINO-TERMINAL PORTION IS CRUCIAL FOR NA+ RECOGNITION IN MELIBIOSE TRANSPORT

被引:0
|
作者
HAMA, H [1 ]
WILSON, TH [1 ]
机构
[1] HARVARD UNIV,SCH MED,DEPT CELLULAR & MOLEC BIOL,BOSTON,MA 02115
关键词
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The melibiose carrier of Escherichia coli couples sugar transport to H+, Na+, and Li+, while that of Klebsiella pneumoniae utilizes only H+ and Li+. We made five chimeric carriers derived from the two carriers to identify the region(s) involved in Na+ recognition. The chimeric carriers E2K10, E4K8, E6K6, E8K4, and E10K2 have the amino-terminal 77, 144, 197, 298, and 349 amino acid residues derived from E. coli and the rest derived from K. pneumoniae, respectively. Melibiose accumulation through the chimeric carriers E2K10, E4K8, and E6K6 was strongly stimulated by Na+ and Li+ as is the case with the E. coli carrier. On the other hand, there was very little stimulation with the carriers E8K4 and E10K2. These results suggest that, 1) the amino-terminal 77 amino acids of the E. coli carrier, which has 5 different and 4 fewer amino acids than the K. pneumoniae carrier, have a crucial role in Na+ recognition in melibiose transport and 2) the carboxyl-terminal half of the carrier also forms a part of the Na+ recognition site which may be distorted in chimeric structures. In contrast with melibiose accumulation, there was very little Na+ stimulation of TMG (methyl-1-thio-beta-D-galactopyranoside) transport and no Na+ stimulation was observed in lactose transport with any of the chimeric carriers, whereas in E. coli Na+ stimulates TMG and lactose transport. These results suggest that there is no universal Na+ recognition site for all the sugar substrates. Instead different parts of the carrier seem to participate in cation recognition for different sugar substrates.
引用
收藏
页码:10060 / 10065
页数:6
相关论文
共 1 条
  • [1] INTERACTION BETWEEN CATIONS AND MELIBIOSE TRANSPORT CARRIER OF CATION-COUPLING MUTANTS OF ESCHERICHIA-COLI
    AKIZAWA, Y
    KAWAKAMI, T
    TSUCHIYA, T
    JOURNAL OF PHARMACOBIO-DYNAMICS, 1986, 9 (09): : S104 - S104