PURIFICATION OF RUBELLA-VIRUS BY ISOPYCNIC GRADIENTS - CONTINUOUS PERCOLL VERSUS DISCONTINUOUS SUCROSE

被引：3

作者：

BUSTOS, J ^{[1
]}

ZAMORA, P ^{[1
]}

MEJIA, E ^{[1
]}

VARELA, Y ^{[1
]}

GOMEZ, B ^{[1
]}

机构：

[1] NATL AUTONOMOUS UNIV MEXICO,FAC MED,LAB VIROL EDIFICIO A SEGUNDO PISO,MEXICO CITY 04510,DF,MEXICO

来源：

ARCHIVES OF VIROLOGY | 1991年 / 118卷 / 3-4期

关键词：

D O I：

10.1007/BF01314039

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Concentrated viral suspensions were purified by isopycnic gradients: discontinuous sucrose, continuous Percoll. With Percoll, homogeneous buoyant density virus were obtained; hemagglutinating titer, concentration factor, specific activity, and reproducible yield were higher than with sucrose.

引用

页码：285 / 288

页数：4

共 31 条

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ARROWOOD, MJ
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PREPARATIVE BIOCHEMISTRY, 1982, 12 (03): : 229 - 234
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JOURNAL OF VIROLOGICAL METHODS, 1991, 31 (2-3) : 161 - 170
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MEDECINE ET MALADIES INFECTIEUSES, 1983, 13 (02): : 79 - 83
[7] THE PURIFICATION OF RUBELLA-VIRUS (RV) AND DETERMINATION OF ITS POLYPEPTIDE COMPOSITION
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JOURNAL OF VIROLOGICAL METHODS, 1985, 10 (03) : 261 - 268
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