PLASMID STABILITY IN IMMOBILIZED MIXED CULTURES OF RECOMBINANT ESCHERICHIA-COLI

被引：18
|
作者：
DINCBAS, V
HORTACSU, A
CAMURDAN, A
机构：
[1] BOGAZICI UNIV,DEPT CHEM ENGN,ISTANBUL 80815,TURKEY
[2] BOGAZICI UNIV,DEPT BIOL,ISTANBUL 80815,TURKEY
来源：
BIOTECHNOLOGY PROGRESS | 1993年 / 9卷 / 02期
关键词：
D O I：
10.1021/bp00020a017
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The growth behavior of a mixed culture of plasmid-free and plasmid-containing (YEp352) Escherichia coli HB101 cells in free suspension and immobilized conditions was studied experimentally. It was found that immobilization in calcium alginate beads delayed the reduction of the plasmid-containing fraction for a period of time inversely related to the total number of cells initially immobilized. A simple unstructured model that assumes compartmentalization of cells in the immobilization matrix at the beginning of growth gave a fit which was in good agreement with the data.
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页码：218 / 220
页数：3
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