DETECTION OF FREE CYTOPLASMIC CIRCLES OF TRANSPOSON TN9 MULTIMERS IN ESCHERICHIA-COLI

被引：0

作者：

SARKARI, JF ^{[1
]}

MAHAJAN, SK ^{[1
]}

机构：

[1] BHABHA ATOM RES CTR,DIV MOLEC BIOL & AGR,BOMBAY 400085,INDIA

来源：

MOLECULAR BIOLOGY REPORTS | 1990年 / 14卷 / 04期

关键词：

TRANSPOSON TN9; CYTOPLASMIC CIRCLES; DNA AMPLIFICATION DEAMPLIFICATION;

D O I：

10.1007/BF00429889

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Extrachromosomal circular DNA molecules consisting of IS1-cat repeats, (IS1-cat)n, were isolated from an E. coli strain harboring nearly 30 copies of tandemly amplified transposon Tn9 located on the chromosome. The DNA 'circles' were characterized by restriction analysis followed by Southern blotting and electron microscopic examination. Their size varied from approximately 5.5 kb to 53 kb.

引用

页码：223 / 229

页数：7

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