QUANTITATIVE-ANALYSIS OF HYALURONAN IN VITREOUS-HUMOR USING CAPILLARY ELECTROPHORESIS

被引：46
|
作者：
GRIMSHAW, J
KANE, A
TROCHAGRIMSHAW, J
DOUGLAS, A
CHAKRAVARTHY, U
ARCHER, D
机构：
[1] DEPT AGR NO IRELAND,DIV VET SCI,STORMONT,ANTRIM,NORTH IRELAND
[2] QUEENS UNIV BELFAST,DEPT OPHTHALMOL,BELFAST,ANTRIM,NORTH IRELAND
来源：
ELECTROPHORESIS | 1994年 / 15卷 / 07期
关键词：
D O I：
10.1002/elps.11501501137
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Capillary electrophoresis was used for the separation and quantitative analysis of the glycosaminoglycan hyaluronan in human and bovine vitreous with detection by W absorbance at 200 nm. Calibration was carried out using standards made up from known concentrations of hyaluronan of umbilical cord origin. The purity of the standard was examined by H-1 NMR (nuclear magnetic resonance). Concentrations as low as 25 mu g/mL could be detected by capillary electrophoresis. Confirmation that the signal was due to hyaluronan was obtained by depolymerization of the native mucopolysaccharide by hyaluronidase. This resulted in loss of the hyaluronan peak and appearance of several new peaks corresponding to the oligomeric fragments which had shorter migration times. Capillary electrophoresis is a reproducible and sensitive technique for the quatification and characterisation of hyaluronan in vitreous samples.
引用
收藏
页码：936 / 940
页数：5
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