Etiology and Antimicrobial Susceptibility Pattern of Bacterial Agents from Urinary Tract Infection in a Tertiary Care Centre

Introduction: The term urinary tract infection (UTI) encompasses a range of infection from cystitis (bladder) to the infection of the entire urinary tract, including the renal pelvis and kidney. Among the infections, UTI is the one of the most important infections in humans. Treatment of urinary tract infection (UTI) was determined by antibiotic susceptibility pattern of bacteria in a population. Objective: The objective of the study is to isolate the common uropathogens in a population and to know the antibiogram for the appropriate empirical treatment. Materials and Methods: A total 135 patient's clean-catch mid-stream urine samples were collected. Samples were cultured onto blood agar and McConkey agar by standard loop method. Isolated bacteria identified by biochemical tests and antibiotic susceptibility testing were done in Muller Hinton Agar by Kirby Bauer method. Result: Among this, 77 culture was positive and showed significant bacteriurea. The predominant bacteria was Escherichia coli (33.8%) followed by Klebsiella sp. (18.2%), Enterococcus sp. (18.2%), Acinetobacter sp. (9.1%), Pseudomonas aeruginosa (7.8%) and Candida sp. (5.2%). All Gram-negative bacteria were resistant to ampicillin and sensitive to tobramycin, amikacin and nitrofurentoin. Conclusion: Gram-negative bacteria were the major isolates in our study, and E. coli was the predominant uropathogen isolated. The knowledge of antibiotic sensitivity pattern in this study will be helpful for the effective treatment of the UTI in this population.