CONFORMATIONAL-CHANGES OF THE PHOSPHATIDYLCHOLINE HEADGROUP DUE TO MEMBRANE DEHYDRATION - A H-2-NMR STUDY

被引：112

作者：

BECHINGER, B ^{[1
]}

SEELIG, J ^{[1
]}

机构：

[1] UNIV BASEL,BIOCTR,DEPT BIOPHYS CHEM,KLINGELBERGSTR 70,CH-4056 BASEL,SWITZERLAND

来源：

CHEMISTRY AND PHYSICS OF LIPIDS | 1991年 / 58卷 / 1-2期

关键词：

DEUTERIUM NMR; P-31; NMR; LIPID HYDRATION; LIPID HEADGROUP; MODEL MEMBRANES; PHOSPHOCHOLINE DIPOLE;

D O I：

10.1016/0009-3084(91)90105-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The influence of hydration on the orientation of the phosphocholine dipole in bilayer membranes was studied with nuclear magnetic resonance. The phosphocholine headgroup of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) was deuterated at the two methylene segments. Phosphorus-31 and deuterium nuclear magnetic resonance measurements were made as a function of hydration in the range of 10-70 wt.% H2O revealing a distinct change in the alignment of the phosphocholine headgroup. With decreasing hydration the N+ end of the phosphocholine head group dipole moves closer to the hydrocarbon layer. The conformational change induced by the loss of water molecules at the membrane surface is qualitatively similar to that observed upon addition of polyhydroxyl compounds.

引用

页码：1 / 5

页数：5

共 31 条

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