CHARACTERIZATION OF VIRULENT AND BENIGN STRAINS OF BACTEROIDES-NODOSUS

被引:34
|
作者
DEPIAZZI, LJ
RICHARDS, RB
HENDERSON, J
ROOD, JI
PALMER, M
PENHALE, WJ
机构
[1] DEPT AGR,ANIM HLTH LAB,S PERTH,WA 6251,AUSTRALIA
[2] DEPT AGR,REG VET LAB,ALBANY,WA 6330,AUSTRALIA
[3] MONASH UNIV,DEPT MICROBIOL,CLAYTON,VIC 3168,AUSTRALIA
[4] MURDOCH UNIV,SCH VET STUDIES,DIV VET BIOL,MURDOCH,WA 6150,AUSTRALIA
关键词
D O I
10.1016/0378-1135(91)90051-G
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The extracellular proteases of 395 isolates of B. nodosus from ovine, bovine and caprine foot lesions were classified as either thermostable or thermolabile. Stable protease was associated with one and unstable protease with four distinctive isoenzyme patterns, each pattern differentiated by the relative mobility of paired isoenzymes. Pathogenicity tests on 64 isolates showed a correlation between the production of stable protease and the production of virulent ovine footrot lesions. The mean values for total protease activity, twitching motility and colony diameter were significantly higher for virulent compared to benign isolates, but the range of values overlapped. SDS-PAGE whole-cell electrophoretic profiles of virulent isolates were similar to the profiles of some benign isolates.
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页码:151 / 160
页数:10
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