OPTIMIZED AMPLIFICATION OF THE POLYMORPHIC SYSTEM COL2A1

被引：2
|
作者：
SKOWASCH, K [1 ]
BRINKMANN, B [1 ]
机构：
[1] UNIV MUNSTER,INST RECHTS MED,VON ESMARCH STR 86,W-4400 MUNSTER,GERMANY
来源：
INTERNATIONAL JOURNAL OF LEGAL MEDICINE | 1992年 / 105卷 / 03期
关键词：
VNTR SYSTEM COL2A1; PCR AMPLIFICATION; OPTIMIZATION;
D O I：
10.1007/BF01625175
中图分类号：
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号：
0301 ; 10 ;
摘要：
The influence of various amplification parameters on the demonstration of COL2A1 patterns was examined in serial experiments. The combination of 6 optimized parameters (concentration of primers, nucleotides, Taq polymerase, K+, Mg2+, number of cycles) led to an approximately tenfold increase in sensitivity and a decrease in allelic drop-out. In unequal mixtures of DNA from 2 individuals the weakest component was detectable in dilutions down to 1: 20. In a small population sample (n = 120) 10 alleles could be demonstrated.
引用
收藏
页码：185 / 187
页数：3
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