ACETYLCHOLINE-RECEPTORS IN DISSOCIATED NUCLEUS BASALIS OF MEYNERT NEURONS OF THE RAT

被引：11
|
作者：
HARATA, N [1 ]
TATEISHI, N [1 ]
AKAIKE, N [1 ]
机构：
[1] TOHOKU UNIV,SCH MED,DEPT NEUROPHYSIOL,SENDAI,MIYAGI 980,JAPAN
来源：
NEUROSCIENCE LETTERS | 1991年 / 130卷 / 02期
关键词：
RAT; DISSOCIATED MEYNERT NEURON; ACETYLCHOLINE; NICOTINE; CARBAMYLCHOLINE; PATCH-CLAMP;
D O I：
10.1016/0304-3940(91)90385-7
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Electrical and pharmacological properties of acetylcholine (ACh)-induced currents in neurons dissociated from the nucleus basalis of Meynert (nBM) of immature (2-week-old) rats were investigated with the whole-cell mode of the patch-clamp technique. At a holding potential (V(H)) of -50 mV, ACh (10(-4)M) evoked a transient inward current mimicked by nicotine (I(nACh)), followed by a sustained outward current mimicked by carbamylcholine (I(mACh)). The K(D) values were 1.2 x 10(-4) M for I(nACh) and 8.7 x 10(-7) M for I(mACh). The reversal potential of I(mACh) was close to E(K). The I(mACh) was determined to be elicited via the M2 muscarinic receptor, based on the differences in sensitivity to muscarinic antagonists such as pirenzepine and AF-DX-116.
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页码：153 / 156
页数：4
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