AXIAL IMAGING THROUGH AN ABERRATING LAYER OF WATER IN CONFOCAL MICROSCOPY

被引：43
|
作者：
SHEPPARD, CJR
GU, M
机构：
[1] Department of Physical Optics, University of Sydney, Sydney
来源：
OPTICS COMMUNICATIONS | 1992年 / 88卷 / 2-3期
关键词：
D O I：
10.1016/0030-4018(92)90508-O
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Axial imaging in confocal microscopy is investigated by considering the effect of spherical aberration caused by focusing a beam of light deeply into a specimen, which is simplified as a layer of water. It is found that the axial response consists of a series of signals reflected from different interfaces. Aberration compensation by altering the effective tube length of the objective is also studied in order to obtain an optimum axial response. Corresponding experimental results are obtained, which are well in agreement with the theoretical predictions.
引用
收藏
页码：180 / 190
页数：11
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