VECTORS WITH RESTRICTION-SITE BANKS .1. PJRD158, A 3903-BP PLASMID CONTAINING 28 UNIQUE CLONING SITES

被引：40

作者：

DAVISON, J ^{[1
]}

HEUSTERSPREUTE, M ^{[1
]}

MERCHEZ, M ^{[1
]}

BRUNEL, F ^{[1
]}

机构：

[1] INT INST CELLULAR & MOLEC PATHOL, MOLEC BIOL UNIT, 75 AVE HIPPOCRATE, B-1200 BRUSSELS, BELGIUM

来源：

GENE | 1984年 / 28卷 / 03期

关键词：

D O I：

10.1016/0378-1119(84)90148-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A DNA fragment was constructed that contains many unique cloning sites not present in currently used Escherichia coli plasmid cloning vehicles. Insertion of this fragment into a modified version of pBR322 results in an AmpRTetR vector (pJRD158) of 3903 bp [base pairs] containing 28 unique cloning sites, 4 almost unique cloning sites and 8 unassigned unique 6-bp palindromes. The plasmid has the additional advantages of very high copy number and altered incompatibility. The latter permits it to be stably maintained in the same host as pBR322.

引用

页码：311 / 318

页数：8

共 41 条

[1] CONSTRUCTION OF PLASMIDS CARRYING CI GENE OF BACTERIOPHAGE-LAMBDA [J].

BACKMAN, K ;

PTASHNE, M ;

GILBERT, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (11) :4174-4178

[2] A CAUTIONARY NOTE ON THE USE OF CERTAIN RESTRICTION ENDONUCLEASES WITH METHYLATED SUBSTRATES [J].

BACKMAN, K .

GENE, 1980, 11 (1-2) :169-171

[3]

Beggs JD., 1981, ALFRED BENZON SYM S, V16, P383

[4]

BERNARD HU, 1980, GENETIC ENG, V2, P133

[5]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[6]

Bolivar F, 1979, Methods Enzymol, V68, P245

[7] CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .2. MULTIPURPOSE CLONING SYSTEM [J].

BOLIVAR, F ;

RODRIGUEZ, RL ;

GREENE, PJ ;

BETLACH, MC ;

HEYNEKER, HL ;

BOYER, HW ;

CROSA, JH ;

FALKOW, S .

GENE, 1977, 2 (02) :95-113

[8]

BROACH JR, 1979, GENE, V8, P121, DOI 10.1016/0378-1119(79)90012-X

[9] RESTRICTION INSENSITIVITY IN BACTERIOPHAGE-T5 .3. CHARACTERIZATION OF ECORI-SENSITIVE MUTANTS BY RESTRICTION ANALYSIS [J].

BRUNEL, F ;

DAVISON, J .

JOURNAL OF MOLECULAR BIOLOGY, 1979, 128 (04) :527-543

[10] CLONING AND EXPRESSION OF TRYPANOSOMA-BRUCEI KINETOPLAST DNA IN ESCHERICHIA-COLI [J].

BRUNEL, F ;

DAVISON, J ;

THI, VH ;

MERCHEZ, M .

GENE, 1980, 12 (3-4) :223-234

← 1 2 3 4 5 →