INFLUENCE OF DISPLACER PROPERTIES ON DISPLACEMENT CHROMATOGRAPHY OF PROTEINS - A THEORETICAL-STUDY

A theoretical framework has been established for the selection/design of polymeric displacers for displacement chromatography of proteins. This framework is based on the stoichiometric displacement model and Martin's additive theory. With this framework, computer simulations of displacement chromatography have been performed to establish linkages between separation characteristics and properties of the displacer. Specifically, the performance characteristics of heterogeneous displacers have been compared to homogeneous displacers, and the influence of the number of interaction ligands on displacement performance has been determined. Some of the results have been compared with previous experimental data, and it has been shown that the theoretical framework provides qualitatively useful guidelines for the selection/design of displacers.