LOCALIZATION OF HIGHLY REPETITIVE, SPECIES-SPECIFIC ECORI ELEMENTS FROM LUPINUS-LUTEUS L

被引:0
|
作者
SAKOWICZ, T [1 ]
LUCHNIAK, P [1 ]
机构
[1] UNIV LODZ,DEPT PLANT CYTOL & CYTOCHEM,PL-90237 LODZ,POLAND
来源
FOLIA HISTOCHEMICA ET CYTOBIOLOGICA | 1995年 / 33卷 / 03期
关键词
LUPINUS LUTEUS; REPETITIVE SEQUENCES; IN SITU HYBRIDIZATION; SATELLITE DNA;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using Southern, dot-blot and in sis hybridization, molecular and cytological localization of repetitive Lupinus luteus DNA sequence was shown. Under CsCl gradient centrifugation conditions CG-rich satellite fraction appeared. Dot-blot hybridization clearly indicated that 1070bp repetitive element being a member of previously described EcoR1 fragments family appeared only in the main band. The use of that DNA fragment as an in situ hybridization probe revealed the hybridization signal in the euchromatin area.
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页码:209 / 213
页数:5
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