SHORT-TERM CELL-CULTURE TECHNIQUE FOR OBTAINING CHROMOSOMES IN MARINE AND FRESH-WATER FISH

被引：40
|
作者：
ALVAREZ, MC [1 ]
OTIS, J [1 ]
AMORES, A [1 ]
GUISE, K [1 ]
机构：
[1] UNIV MINNESOTA,DEPT ANIM SCI,ST PAUL,MN 55108
来源：
JOURNAL OF FISH BIOLOGY | 1991年 / 39卷 / 06期
关键词：
FISH CELL CULTURE; FISH CHROMOSOMES;
D O I：
10.1111/j.1095-8649.1991.tb04411.x
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
In this paper we describe a simple and reliable short‐term fish cell culture technique, for obtaining chromosome spreads with minimum stress involving the specimens sampled. Fibroblasts were derived from primary cultures from the caudal fin of some economically important freshwater species: northern pike, Esox lucius L., goldfish, Carassius auratus L., and marine species: gilthead sea bream, Sparus aurata L. and cardinal fish, Apogon imberbisL. Copyright © 1991, Wiley Blackwell. All rights reserved
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页码：817 / 824
页数：8
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