ISOLATION, CHARACTERIZATION, DOCKING AND ANTI-CANCER ACTIVITY OF QUERCETIN FROM LEAVES OF EUPHORBIA HETEROPHYLLA LINN.

This study was carried out to isolate and characterize the bio active constituent from Euphorbia heterophylla and also to evaluate anticancer activity for the isolated compound against Breast cancer cell line (MCF -7 Cell line). The isolation of compound was performed on ethanol extract by using column chromatography and ultra sonicator by gradient elution. The structure of isolated compound was established on the basis of chemical test, thin layer chromatography and spectroscopic evidences. (IR, 1H NMR, C-13 NMR, Liquid chromatography / tandem mass spectrometry (LC/MS/MS identification with data base library search). The yellow colour compound shows positive test for flavonoids in Thin layer Chromatography for flavonoids in the solvent system n-butanol: acetic acid: water (2: 2: 6) with R-f value 0.7. From the use of LCMS spectral library search system and TLC it is confirmed that the isolated compound is found to be quercetin. The Docking study was performed for the isolated quercetin against 5, 10 methenyltetrahydrofolate synthetase (MTHFS). The inhibition of (MTHFS) in human MCF 7 breast cancer cells has been shown to arrest the growth of breast cancer cells with the docking score of -10.23. Hence quercetin was subjected to in vitro anti-cancer activity against MCF-7 cell line. At 48 hrs in 100 mu g/ml, the mean percentage cytotoxicity was found to be 89.77 and the percentage viability was 10.22.