EFFECT OF GAMMA-IRRADIATION ON REACTIVITY OF RINDERPEST VIRUS-ANTIGEN WITH BOVINE IMMUNE SERUM IN ENZYME-LINKED IMMUNOSORBENT ASSAYS AND VIRUS NEUTRALIZATION AND INDIRECT FLUORESCENT-ANTIBODY TESTS

被引：5

作者：

SALIKI, JT ^{[1
]}

BERNINGER, ML ^{[1
]}

TORRES, A ^{[1
]}

HOUSE, JA ^{[1
]}

MEBUS, CA ^{[1
]}

DUBOVI, EJ ^{[1
]}

机构：

[1] CORNELL UNIV,NYS COLL VET MED,DIAGNOST LAB,ITHACA,NY 14851

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1993年 / 31卷 / 02期

关键词：

D O I：

10.1128/JCM.31.2.428-432.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Gamma irradiation effectively inactivated gradient-purified rinderpest virus. Irradiated antigen and sera remained functional in enzyme-linked immunosorbent assays, virus neutralization tests, and indirect fluorescent-antibody tests. Irradiation, however, led to a dose-dependent decrease in reactivity, particularly significant (P < 0.05) when both reagents were irradiated. To avoid false-positive reactions, only one reagent (serum or antigen) may be irradiated.

引用

页码：428 / 432

页数：5

共 48 条

[1] COMPARISON OF 3 ENZYME-LINKED IMMUNOSORBENT ASSAYS AND A DIRECT FLUORESCENT-ANTIBODY TEST FOR DETECTION OF RESPIRATORY SYNCYTIAL VIRUS-ANTIGEN
KRILOV, LR
MARCOUX, L
ISENBERG, HD
JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (02) : 377 - 379
[2] QUANTITATION OF HEMORRHAGIC ENTERITIS VIRUS-ANTIGEN AND ANTIBODY USING ENZYME-LINKED IMMUNOSORBENT ASSAYS
VANDENHURK, JV
AVIAN DISEASES, 1986, 30 (04) : 662 - 671
[3] THE EFFECT ON KAOLIN ADSORPTION OF SERUM OF THE VIRUS NEUTRALIZATION AND ENZYME-LINKED IMMUNOSORBENT ASSAYS OF ANTIBODY TO BOVINE HERPESVIRUS-1
DARCEL, CL
KOZUB, GC
JOURNAL OF BIOLOGICAL STANDARDIZATION, 1984, 12 (01): : 87 - 92
[4] COMPARISON OF 2 COMMERCIAL ENZYME-LINKED IMMUNOSORBENT ASSAYS AND FLUORESCENT-ANTIBODY TO MEMBRANE ANTIGEN TEST FOR IMMUNE STATUS TO VARICELLA ZOSTER VIRUS
LI, TC
RUTHERFORD, I
SERODIAGNOSIS AND IMMUNOTHERAPY IN INFECTIOUS DISEASE, 1988, 2 (06) : 395 - 399
[5] COMPARISON OF 2 ENZYME-LINKED IMMUNOSORBENT ASSAYS FOR DETECTION OF HERPES-SIMPLEX VIRUS-ANTIGEN
GONIK, B
SEIBEL, M
BERKOWITZ, A
WOODIN, MB
MILLS, K
JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (03) : 436 - 438
[6] ENZYME-LINKED IMMUNOSORBENT-ASSAY FOR SERUM ANTIBODY TO BOVINE RESPIRATORY SYNCYTIAL VIRUS - COMPARISON WITH COMPLEMENT-FIXATION AND NEUTRALIZATION TESTS
GILLETTE, KG
AMERICAN JOURNAL OF VETERINARY RESEARCH, 1983, 44 (12) : 2251 - 2255
[7] COMPARISON OF 2 COMMERCIAL ENZYME-LINKED IMMUNOSORBENT ASSAYS FOR DETECTION OF HERPES-SIMPLEX VIRUS-ANTIGEN
WARFORD, AL
LEVY, RA
STRONG, CA
REKRUT, KA
AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1986, 85 (02) : 229 - 232
[8] INDIRECT ENZYME-LINKED IMMUNOSORBENT-ASSAY FOR THE DETECTION OF FOOT-AND-MOUTH-DISEASE VIRUS-ANTIGEN
PATTNAIK, B
VENKATARAMANAN, R
INDIAN JOURNAL OF ANIMAL SCIENCES, 1989, 59 (03): : 317 - 322
[9] EVALUATION OF AN INDIRECT FLUORESCENT-ANTIBODY TEST, ENZYME-LINKED IMMUNOSORBENT ASSAY AND QUANTIFICATION OF IMMUNOGLOBULINS IN DIAGNOSIS OF BOVINE TRYPANOSOMIASIS
LUCKINS, AG
MEHLITZ, D
TROPICAL ANIMAL HEALTH AND PRODUCTION, 1978, 10 (03) : 149 - 159
[10] Diagnosis and clinical virology of Lassa fever as evaluated by enzyme-linked immunosorbent assay, indirect fluorescent-antibody test, and virus isolation
Bausch, DG
Rollin, PE
Demby, AH
Coulibaly, M
Kanu, J
Conteh, AS
Wagoner, KD
McMullan, LK
Bowen, MD
Peters, CJ
Ksiazek, TG
JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (07) : 2670 - 2677

← 1 2 3 4 5 →