KINETIC CHARACTERIZATION OF A MODEL FOR ZYMOGEN ACTIVATION - AN EXPERIMENTAL-DESIGN AND KINETIC DATA-ANALYSIS

被引：7
|
作者：
VAZQUEZ, A
VARON, R
TUDELA, J
GARCIACANOVAS, F
机构：
[1] UNIV MURCIA,DEPT BIOQUIM & BIOL MOLEC,MURCIA,SPAIN
[2] UNIV CASTILLA LA MANCHA,DEPT QUIM FIS,LA MANCHA,SPAIN
来源：
JOURNAL OF MOLECULAR CATALYSIS | 1993年 / 79卷 / 1-3期
关键词：
ENZYME KINETICS; EXPERIMENTAL DESIGN; KINETIC DATA ANALYSIS; PROTEOLYSIS; ZYMOGEN ACTIVATION;
D O I：
10.1016/0304-5102(93)85114-9
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
The kinetic equations of both the transient phase and the steady state of some mechanisms, considered as particular cases of a general model for enzyme activation through limited proteolysis, are obtained. Two alternative experimental approaches, an excess of zymogen, or of activating enzyme, are used. Procedures for the evaluation of the kinetic parameters and rate constants are developed and these methods are applied to the simulated data obtained by using a personal computer in order to verify the reliability of the method.
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页码：347 / 363
页数：17
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