SENSITIVE BENEDICT TEST

被引:2
|
作者
CHEN, Q [1 ]
KLEMM, N [1 ]
DUNCAN, G [1 ]
JENG, IM [1 ]
机构
[1] UNIV MISSOURI,SCH MED,DEPT BIOCHEM,MISSOURI INST PSYCHIAT,NEUROCHEM RES UNIT,5400 ARSENAL,ST LOUIS,MO 63139
关键词
2,2′-bicinchoninic acid; Benedict test; Hydroxyaldehyde; Hydroxyketone; Ketoaldehyde;
D O I
10.1039/an9901500109
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The Benedict reaction is a versatile test for the detection of easily oxidised compounds. The use of 2,2′-bicinchoninicacid to detect the production of copper(I) in the Benedict test is described. This produced a test that is more sensitive than the classical Benedict test. A sensitive spot test for Benedict-positive compounds and a spray for compounds on thin-layer chromatographic plates is also described. Compounds are classified into three categories according to their sensitivity to the proposed Benedict test.
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页码:109 / 110
页数:2
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