QUANTIFICATION OF CHLORHEXIDINE GLUCONATE FROM MOUTH RINSE AND SKIN DISINFECTANT SOLUTIONS USING A VAIDATED CHROMATOGRAPHIC TECHNIQUE

The study presents a simple and rapid chromatographic technique for the estimation of chlorhexidine gluconate for mouth wash using a combination of phosphate buffer and acetonitrile as mobile phase. The separation was carried out on a stainless steel column 250mm x 4.5mm packed with octadecyle silane particles. The detection was carried out using a dual lambda absorbance detector at 254nm wavelength. The method is found to be linear in the concentration range 10.56 mu g/ml to 98.56 mu g/ml and correlation coefficient 0.998. The analyte peak was found to be symmetric and the average retention time is 2.919mins. The method was found to selective, precise and accurate for the quantification of the drug from mouth rinse available in the market.