MONOLAYERS OF PHOTOSYNTHETIC REACTION CENTERS OF GREEN AND PURPLE BACTERIA

被引:22
|
作者
ZAITSEV, SY
KALABINA, NA
ZUBOV, VP
LUKASHEV, EP
KONONENKO, AA
UPHAUS, RA
机构
[1] MV LOMONOSOV STATE UNIV,DEPT BIOPHYS,MOSCOW 119899,USSR
[2] IOWA STATE UNIV SCI & TECHNOL,AMES LAB,AMES,IA 50011
关键词
D O I
10.1016/0040-6090(92)90385-O
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
Monolayers of the reaction centers isolated from the photosynthetic bacteria Chloroflexus aurantiacus, Rhodobacter sphaeroides and Rhodopseudomonas viridis were prepared and investigated. The monolayer characteristics of these proteins were analyzed to determine the optimum conditions for stable film preparations. Monolayers were transferred onto quartz and metal supports by the Langmuir-Schaefer method to provide multilayers of definite thickness. These films retain the optical and photoelectrical properties of the native photosynthetic membranes. The sign of the photopotential provides evidence for a different orientation of the reaction centers of Rb. sphaeroides compared to those of Rps. viridis.
引用
收藏
页码:723 / 725
页数:3
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