Molecular species of the major phospholipids, phosphatidylcholine (PC, 49%of total phospholipids), phosphatidylethanolamine (PE, 27 %), and phosphatidylinositol (PI, 11 %), of Lilium longiflorum pollen grains were analyzed. Fatty acid compositions (sn-l/sn-2) of major molecular species of PC, PE, and PI were 18: 3/18: 3, 18: 2/18: 3 (and/or 18: 3/18: 2), 18: 2/18: 2, 16: 0/18: 3, and 16: 0/18: 2. Molecular species compositions were quite similar among PC, PE and PI. Mature pollen grains contained more unsaturated molecular species than immature pollen grains. © 1990 by the Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.