A METHOD FOR DETECTION OF AROMATIC METABOLITES AT VERY-LOW CONCENTRATIONS - APPLICATION TO DETECTION OF METABOLITES OF ANAEROBIC TOLUENE DEGRADATION

被引：10
|
作者：
EDWARDS, EA
EDWARDS, AM
GRBICGALIC, D
机构：
[1] STANFORD UNIV,DEPT CIVIL ENGN,STANFORD,CA 94305
[2] MCMASTER UNIV,DEPT PATHOL,HAMILTON L8N 2Z5,ON,CANADA
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1994年 / 60卷 / 01期
关键词：
D O I：
10.1128/AEM.60.1.323-327.1994
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Difficulties inherent in working with anaerobic microorganisms and mixed cultures have hampered efforts to detect and identify metabolites of anaerobic degradation of monoaromatic compounds. Isotope-trapping experiments and analysis using a high-performance liquid chromatograph equipped with a flow-through radioactivity detector were used to detect very low concentrations of metabolites. Data obtained by this method suggest that toluene was degraded via methyl hydroxylation by a mixed methanogenic culture.
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页码：323 / 327
页数：5
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