A RAPID CHEMILUMINESCENT DETECTION METHOD FOR BARLEY YELLOW DWARF VIRUS

被引：18
|
作者：
FOULY, HM
DOMIER, LL
DARCY, CJ
机构：
[1] UNIV ILLINOIS,DEPT PLANT PATHOL,1102 S GOODWIN AVE,URBANA,IL 61801
[2] UNIV ILLINOIS,USDA ARS,CROP PROTECT RES UNIT,URBANA,IL 61801
来源：
JOURNAL OF VIROLOGICAL METHODS | 1992年 / 39卷 / 03期
关键词：
LUTEOVIRUS; DOT BLOT; HYBRIDIZATION;
D O I：
10.1016/0166-0934(92)90102-J
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Barley yellow dwarf virus (BYDV-PAV-IL) was detected with biotinylated in vitro transcript cDNA using a chemiluminescent substrate on nylon membranes. Signals were detected on X-ray film and quantified using either a densitometer or an ELISA plate reader. The time required for sample preparation was reduced so that the entire protocol could be completed in two days. The in vitro transcript probes could detect 1 ng of purified virus and as little as 1 mul of sap extracts prepared from infected oat shoots.
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页码：291 / 298
页数：8
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