ANALYSIS OF TROPANE ALKALOIDS WITH THERMOSPRAY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY

被引：26
|
作者：
AURIOLA, S [1 ]
MARTINSEN, A [1 ]
OKSMANCALDENTEY, KM [1 ]
NAARANLAHTI, T [1 ]
机构：
[1] UNIV HELSINKI, DEPT PHARM, DIV PHARMACOGNOSY, SF-00170 HELSINKI 17, FINLAND
来源：
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 562卷 / 1-2期
关键词：
D O I：
10.1016/0378-4347(91)80622-J
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A thermospray high-performance liquid chromatography-mass spectrometry method for analysis of hyoscyamine and scopolamine in plant cell culture samples is described. The alkaloids were separated on a polymeric reversed-phase column with an alkaline ammonium acetate buffer-acetonitrile eluent. Selected-ion recording of the protonated molecular ions was used for quantitation of the compounds. The compounds were fragmented by discharge-assisted ionization and elevated thermospray capillary temperatures or ion repeller potentials.
引用
收藏
页码：737 / 744
页数：8
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