Application of rhodamine-based fluorescent molecular probes in visualization of cellular pyruvic acid

被引：0

作者：

Wang Shuo ^{[1
,2
]}

Sun Xiaoyan ^{[3
]}

Chen Jinlong ^{[1
,2
]}

机构：

[1] China Pharmaceut Univ, Sch Pharm, Dept Pharmaceut Anal, Nanjing 210009, Jiangsu, Peoples R China

[2] China Pharmaceut Univ, Minist Educ, Key Lab Drug Qual Control & Pharmacovigilance, Nanjing 210009, Jiangsu, Peoples R China

[3] Jiangsu Prov Inst Chinese Med, Lab Cellular & Mol Biol, Nanjing 210028, Jiangsu, Peoples R China

来源：

JOURNAL OF CHINA PHARMACEUTICAL UNIVERSITY | 2018年 / 49卷 / 01期

关键词：

pyruvic acid; fluorescent probe; rhodamine; 6G; fluorescence imaging;

D O I：

10.11665/issn.1000-5048.20180111

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

In order to conduct the visualized research of cellular pyruvic acid (PA), several rhodamine-based probes were designed to react with PA by a Schiff-base reaction in aqueous media and exhibit strong fluorescence emission and a pink color owing to the ring-opening of the corresponding spirolactam. The results showed that R6G-NH2 had a highest sensitive response to PA with a linearly relationship between the enhancement of fluorescence intensity at 565 nm and the concentration of PA from 0.2 mu mol/L to 120 mu mol/L. According to the principle of 3 sigma, a limit of detection was obtained as low as 0.1 mu mol/L. Furthermore, the response of R6G-NH2 to PA was hardly affected by conventional biomolecules and ionic species. Therefore, R6G-NH2 was successfully used in fluorescence imaging of exogenous PA in non-small cell lung cancer cell A549 and renal epithelial cell 293T, and endogenous PA induced by bisphenol A in epithelial cell 293T.

引用

页码：79 / 86

页数：8