PSEUDOMONAS-AERUGINOSA ALIPHATIC AMIDASE IS RELATED TO THE NITRILASE CYANIDE HYDRATASE ENZYME FAMILY AND CYS(166) IS PREDICTED TO BE THE ACTIVE-SITE NUCLEOPHILE OF THE CATALYTIC MECHANISM

被引：50

作者：

NOVO, C

TATA, R

CLEMENTE, A

BROWN, PR

机构：

[1] UNIV LONDON KINGS COLL, DIV BIOMED SCI, MOLEC BIOL & BIOPHYS SECT, LONDON WC2R 2LS, ENGLAND

[2] INETI, IBQTA, P-2745 QUELUZ, PORTUGAL

来源：

FEBS LETTERS | 1995年 / 367卷 / 03期

关键词：

AMIDASE; NITRILASE; PHYLOGENETIC TREE; ACTIVE SITE PREDICTION; PSEUDOMONAS AERUGINOSA; RHODOCOCCUS ERYTHROPOLIS;

D O I：

10.1016/0014-5793(95)00585-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A database search indicated homology between some members of the nitrilase/cyanide hydratase family, Pseudomonas aeruginosa and Rhodococcus erythropolis amidases and several other proteins, some of unknown function, BLOCK and PROFILE searches confirmed these relationships and showed that four regions of the P. aeruginosa amidase had significant homology with corresponding regions of nitrilases. A phylogenetic tree placed the P. aeruginosa and R. erythropolis amidases in a group with nitrilases but separated other amidases into three groups, The active site cysteine in nitrilases is conserved in the P. aeruginosa amidase indicating that Cys(166) is the active site nucleophile.

引用

页码：275 / 279

页数：5

共 2 条

[1] Evidence that cysteine-166 is the active-site nucleophile of Pseudomonas aeruginosa amidase: Crystallization and preliminary X-ray diffraction analysis of the enzyme
Farnaud, Sebastien
Tata, Renee
Sohi, Maninder K.
Wan, Tommy
Brown, Paul R.
Sutton, Brian J.
Biochemical Journal, 340 (03): : 711 - 714
[2] Evidence that cysteine-166 is the active-site nucleophile of Pseudomonas aeruginosa amidase:: crystallization and preliminary X-ray diffraction analysis of the enzyme
Farnaud, S
Tata, R
Sohi, MK
Wan, T
Brown, PR
Sutton, BJ
BIOCHEMICAL JOURNAL, 1999, 340 : 711 - 714

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