A REGULATORY ELEMENT IN THE 5'UTR DIRECTS CELL-SPECIFIC EXPRESSION OF THE MOUSE ALPHA(4) GENE

被引：8
|
作者：
SCHOLLEN, E [1 ]
DEMEIRSMAN, C [1 ]
MATTHIJS, G [1 ]
CASSIMAN, JJ [1 ]
机构：
[1] CATHOLIC UNIV LEUVEN, CTR HUMAN GENET, B-3000 LOUVAIN, BELGIUM
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 211卷 / 01期
关键词：
D O I：
10.1006/bbrc.1995.1785
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Transfection experiments showed that the mouse alpha(4) promoter contains a downstream element in its 5'UTR which is essential for efficient promoter activity. DNaseI footprinting and electrophoretic mobility shift assays (EMSA) demonstrated that the region from nt +113 to +148 can bind a cell type-specific factor (MIII-3) present in the alpha(4)m expressing cell line 1,L1210 but not in the non-expressing cell lines A9 and LMTK(-). Two consensus SP1 sites in this 5'UTR were recognised in L1210, A9 and LMTK(-), and with extracts from A9 and LMTK(-), an AP2-like protein was shown to bind a downstream AP2 site. Thus both ubiquitous and cell type-specific factors regulate the expression of alpha(4)m. (C) 1995 Academic Press, Inc.
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收藏
页码：115 / 122
页数：8
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