PRODUCTION OF FERULOYL RHO-COUMAROYL ESTERASE-ACTIVITY BY PENICILLIUM-EXPANSUM, PENICILLIUM-BREVICOMPACTUM AND ASPERGILLUS-NIGER

被引:20
|
作者
DONAGHY, JA [1 ]
MCKAY, AM [1 ]
机构
[1] QUEENS UNIV BELFAST,BELFAST BT7 1NN,ANTRIM,NORTH IRELAND
来源
JOURNAL OF APPLIED BACTERIOLOGY | 1995年 / 79卷 / 06期
关键词
D O I
10.1111/j.1365-2672.1995.tb00951.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Extracellular esterase production by Penicillium expansum, Penicillium brevicompactum and Aspergillus niger was determined in both liquid and solid-state culture. Methyl ferulate was used as the main carbon source in liquid culture whereas wheat bran and sugar beet pulp were used in solid-state culture. Extracted enzyme for each fungus showed activity in the presence of ONP butyrate, methyl ferulate, methyl coumarate and two 'natural' feruloylated carbohydrate esters. Higher enzyme recoveries were obtained using wheat bran in solid-state culture. Higher levels of feruloyl esterase activity were recovered from P. expansum on all feruloylated substrates than from P. brevicompactum or A. niger. Using ONP butyrate as substrate the pH and temperature optima for the esterases of both Penicillium spp. were 6.0 and 25-30 degrees C. Aspergillus niger esterase activity showed a broader temperature range with an optimum at 40 degrees C.
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页码:657 / 662
页数:6
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