AN AUTOMATIC PULSED LASER MICROFLUOROMETER WITH HIGH SPATIAL AND TEMPORAL RESOLUTION

An automatic pulsed laser microfluorometer was described with high spatial and temporal resolution. The instrument consists of: a nitrogen-laser-pumped dye-laser for the excitation of the fluorescence, a microscope with additional optics to focus the excitation beam on the sample and to collect the fluorescence, filters or monochromators to select the output wavelength, a fast photomultiplier tube to detect the signal, and a dual time-scale microprocessor-controlled signal averager for the acquisition and processing of the signal. The potential of the time-resolved fluorescence microscopy in studying, quantitatively and qualitatively, the properties of fluorescent molecules was given.