CLONING AND TRANSIENT EXPRESSION OF GENES ENCODING THE HUMAN ALPHA-4 AND BETA-2 NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTOR (NACHR) SUBUNITS

被引：31

作者：

MONTEGGIA, LM

GOPALAKRISHNAN, M

TOUMA, E

IDLER, KB

NASH, N

ARNERIC, SP

SULLIVAN, JP

GIORDANO, T

机构：

[1] ABBOTT LABS,ABBOTT PK,IL 60064

[2] SCIOS NOVA INC,BALTIMORE,MD 21224

来源：

GENE | 1995年 / 155卷 / 02期

关键词：

ION CHANNEL; POLYMERASE CHAIN REACTION; CDNA; SEQUENCE HOMOLOGY; CYTISINE;

D O I：

10.1016/0378-1119(94)00914-E

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Partial cDNA clones generated by RT-PCR were used as probes to clone the cDNAs encoding the human alpha 4 and beta 2 neuronal nicotinic acetylcholine receptor (nAChR) subunits. The 2.1-kb alpha 4 cDNA shows 84 and 76% identity to the rat and chicken cDNA sequences, respectively, The deduced amino-acid sequence shares 89 and 84% similarity, respectively, with the corresponding rat and chicken proteins, with most of the divergence occurring in the cytoplasmic domain, The 1721-nucleotide beta 2 sequence was identical to the human beta 2 sequence previously reported. Transfection of the alpha 4 and beta 2 clones into HEK293 cells resulted in the formation of binding sites that display high affinity towards [H-3] cytisine, a characteristic of the alpha 4 beta 2 subtype produced in vivo.

引用

页码：189 / 193

页数：5

共 50 条

[1] CHROMOSOMAL LOCALIZATION OF THE MOUSE GENES-CODING FOR ALPHA-2, ALPHA-3, ALPHA-4 AND BETA-2 SUBUNITS OF NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTOR
BESSIS, A
SIMONCHAZOTTES, D
DEVILLERSTHIERY, A
GUENET, JL
CHANGEUX, JP
FEBS LETTERS, 1990, 264 (01) : 48 - 52
[2] EXPRESSION OF NEURONAL ACETYLCHOLINE NICOTINIC RECEPTOR ALPHA-4 AND BETA-2 SUBUNITS DURING POSTNATAL-DEVELOPMENT OF THE RAT-BRAIN
CIMINO, M
MARINI, P
COLOMBO, S
ANDENA, M
CATTABENI, F
FORNASARI, D
CLEMENTI, F
JOURNAL OF NEURAL TRANSMISSION-GENERAL SECTION, 1995, 100 (02) : 77 - 92
[3] CELLULAR-DISTRIBUTION IN THE RAT TELENCEPHALON OF MESSENGER-RNAS ENCODING FOR THE ALPHA-3 AND ALPHA-4 SUBUNITS OF THE NICOTINIC ACETYLCHOLINE-RECEPTOR
LOBRON, C
WEVERS, A
DAMGEN, K
JESKE, A
RONTAL, D
BIRTSCH, C
HEINEMANN, S
REINHARDT, S
MAELICKE, A
SCHRODER, H
MOLECULAR BRAIN RESEARCH, 1995, 30 (01): : 70 - 76
[4] EXPRESSION OF ALPHA-4 AND BETA-2 NICOTINIC ACETYLCHOLINE-RECEPTOR SUBUNIT MESSENGER-RNA AND LOCALIZATION OF ALPHA-BUNGAROTOXIN BINDING-PROTEINS IN THE RAT VESTIBULAR PERIPHERY
WACKYM, PA
POPPER, P
LOPEZ, I
ISHIYAMA, A
MICEVYCH, PE
CELL BIOLOGY INTERNATIONAL, 1995, 19 (04) : 291 - 300
[5] DEVELOPMENTAL EXPRESSION OF THE GENES ENCODING THE 4 SUBUNITS OF THE CHICKEN MUSCLE ACETYLCHOLINE-RECEPTOR
MOSS, SJ
DARLISON, MG
BEESON, DMW
BARNARD, EA
JOURNAL OF BIOLOGICAL CHEMISTRY, 1989, 264 (34) : 20199 - 20205
[6] Cloning and sequence of full-length cDNAs encoding the human neuronal nicotinic acetylcholine receptor (nAChR) subunits beta 3 and beta 4 and expression of seven nAChR subunits in the human neuroblastoma cell line SH-SY5Y and/or IMR-32
GrootKormelink, PJ
Luyten, WHML
FEBS LETTERS, 1997, 400 (03) : 309 - 314
[7] Assembly of human neuronal nicotinic receptor alpha 5 subunits with alpha 3, beta 2, and beta 4 subunits
Wang, F
Gerzanich, V
Wells, GB
Anand, R
Peng, X
Keyser, K
Lindstrom, J
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (30) : 17656 - 17665
[8] Comparative structure of human neuronal alpha 2-alpha 7 and beta 2-beta 4 nicotinic acetylcholine receptor subunits and functional expression of the alpha 2, alpha 3, alpha 4, alpha 7, beta 2, and beta 4 subunits
Elliott, KJ
Ellis, SB
Berckhan, KJ
Urrutia, A
ChavezNoriega, LE
Johnson, EC
Velicelebi, G
Harpold, MM
JOURNAL OF MOLECULAR NEUROSCIENCE, 1996, 7 (03) : 217 - 228
[9] CLONING AND MAPPING OF THE MOUSE ALPHA-7-NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTOR
ORRURTREGER, A
SELDIN, MF
BALDINI, A
BEAUDET, AL
GENOMICS, 1995, 26 (02) : 399 - 402
[10] NUCLEOTIDE-SEQUENCE OF THE HUMAN NICOTINIC ACETYLCHOLINE-RECEPTOR BETA-2 SUBUNIT GENE
ANAND, R
LINDSTROM, J
NUCLEIC ACIDS RESEARCH, 1990, 18 (14) : 4272 - 4272

← 1 2 3 4 5 →