REGULATION OF THE EXPRESSION OF TYPE-I COLLAGEN GENES - IMPLICATIONS FOR THE STUDY OF SCLERODERMA

Type I collagen, the most abundant protein of the body, is preferentially synthesized in bone, dermis, and tendons by two cell types, the osteoblast and the fibroblast. The expression of type I collagen is increased in the various forms of fibrosis such as lung, liver, bone marrow fibrosis and scleroderma. Type I collagen is a heterotrimer molecule consisting of two alpha(I) chains and one alpha 2(I) chain. The two polypeptide chains are synthesized in a 2:1 stoichiometry. The same 2:1 ratio is observed for the rate of synthesis of the corresponding mRNAs. One hypothesis that would explain how this coregulation occurs at the transcriptional level is that common cis-acting elements are present on both genes. These common regulatory elements would identical transcription factors playing the same function. The characterization of the various regulatory elements present in these genes would foster our understanding of the molecular mechanisms controlling type I collagen genes expression in normal and in pathological situations. Over the past few years, several laboratories have identified cis-acting elements in the promoters of the colla1 and colla2 genes. At least, two of these cis-acting elements are common to both promoters. One is centered by a pentanucleotide CCAAT and binds a ubiquituously expressed heteromeric CCAAT binding factor. A. second one is centered by a G-rich region and it binds a new transription factor called C-Krox. Interestingly, C-Krox gene, whose expression is regulated by growth factors, is preferentially expressed in skin fibroblasts in mice and is absent in bone suggesting that it plays a role in the fibroblast specific expression of type I collagen genes. The knowledge of how this and eventually other transcription factors act to regulate collagen expression will eventually lead to a better understanding of the increased type I collagen gene expression seen in diseases such as scleroderma.