THE ROLE OF THE LIVER IN THE PRODUCTION OF FREE-RADICALS DURING HALOTHANE ANESTHESIA IN THE RAT - QUANTIFICATION OF N-TERT-BUTYL-ALPHA-(4-NITROPHENYL)NITRONE (PBN)-TRAPPED ADDUCTS IN BILE FROM HALOTHANE AS COMPARED WITH CARBON-TETRACHLORIDE

被引：14

作者：

HUGHES, HM ^{[1
]}

GEORGE, IM ^{[1
]}

EVANS, JC ^{[1
]}

ROWLANDS, CC ^{[1
]}

POWELL, GM ^{[1
]}

CURTIS, CG ^{[1
]}

机构：

[1] UNIV WALES COLL CARDIFF,COLL CARDIFF,SCH CHEM & APPL CHEM,CARDIFF CF1 1ST,WALES

来源：

BIOCHEMICAL JOURNAL | 1991年 / 277卷

关键词：

D O I：

10.1042/bj2770795

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Halothane or CCl4 was co-administered with the spin trap N-tert-butyl-alpha-(4-nitrophenyl)nitrone (PBN) to rats fitted with bile duct cannuli or to isolated perfused liver preparations. Rats maintained under halothane anaesthesia generated significant amounts of free radicals, and 5-9 nmol was excreted in bile over 1 h. No adducts were detected in urine or plasma. The hepatic origin of these free radicals was confirmed by studies on isolated perfused livers where the addition of halothane to the perfusate resulted in the biliary elimination of the same PBN-trapped radical adducts. Similarly, following CCl4 administration, the same radical species were eliminated in bile in the whole animal and the perfused liver preparation. In the perfused liver, over 3 h the total biliary elimination of radicals derived from halothane or CCl4 (administered at equimolar concentrations) was approximately the same (5-7 nmol); however, the elimination of halothane-derived radicals was more rapid over the first 1 h.

引用

页码：795 / 800

页数：6