PROTEIN HYDRATION IN AQUEOUS-SOLUTION

被引：752

作者：

OTTING, G

LIEPINSH, E

WUTHRICH, K

机构：

[1] Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule-Hönggerberg

来源：

SCIENCE | 1991年 / 254卷 / 5034期

关键词：

D O I：

10.1126/science.1948083

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

High-resolution proton nuclear magnetic resonance studies of protein hydration in aqueous solution show that there are two qualitatively different types of hydration sites. A well-defined, small number of water molecules in the interior of the protein are in identical locations in the crystal structure and in solution, and their residence times are in the range from about 10(-2) to 10(-8) second. Hydration of the protein surface in solution is by water molecules with residence times in the subnanosecond range, even when they are located in hydration sites that contain well-ordered water in the x-ray structures of protein single crystals.

引用

页码：974 / 980

页数：7

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