IDENTIFICATION AND SETTING LEVEL COUMPOUNDS FOR KUMARIN IN THE METANOL EXTRACT Artemisia Annua L. BY THIN LAYER CHROMATOGRAPHY - DENSITOMETRY

Artemisia annua L. contain the active compounds include: terpenoids, flavonoids, kumarin, artemisinin acid, artennuin B, phenols, saponins, and fat Kumarin and its derivatives have biological activity that can stimulate skin pigment, blood anticoagulation and can inhibit the effects of carcinogens. With this biological activity of kumarin, the research is done to ensure there is kumarin by identification and measure kumarin level which is contained in the Artemisia annua L.herb. The analysis methods include the extraction and fractionation. Identification and determination of level with Thin-Layer Chromatography (TLC) using a Densitometer CS-9301 PC. From the result of TLC identification of kumarin standard known that Artemisia annua L extract contain kumarin compound which marked by a blue spot flouresense on standards and methanol extract of artemisia annua L. seeing under UV light at a wavelength of 366 nm with Rf value of standard and sample is 0.31, the measurement of kumarin spot with Densitometer known that kumarin concentration in the extract of Artemisia annua L. is 10.5 ul / ml with 105% Recovery