EFFECTS OF SOLVENT MOLECULAR TOXICITY AND MICROENVIRONMENT COMPOSITION ON THE DELTA-DEHYDROGENATION ACTIVITY OF ARTHROBACTER-SIMPLEX CELLS

被引：25
|
作者：
PINHEIRO, HM
CABRAL, JMS
机构：
[1] Laboratório de Engenharia Bioquímica, Instituto Superior Técnico, Lisbon
来源：
BIOTECHNOLOGY AND BIOENGINEERING | 1991年 / 37卷 / 02期
关键词：
D O I：
10.1002/bit.260370202
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Solvent selection tests were carried out for the DELTA1 dehydrogenation of 6-alpha-methylhydrocortisone-21-acetate by Arthrobacter simplex cells in the presence of organic solvents. Solubility limits were determined for substrate and product in dry and water-saturated solvents and solvent mixtures. Molecular toxicity levels were estimated by measuring the dehydrogenation activity decay of freely suspended cells incubated in solvent-saturated aqueous media. Chloroform and n-decan-1-ol were the best choice of solvent, for both solubility and catalytic stability. High concentrations of water-soluble additives, such as monosodium glutamate, were found to greatly improve the retention of activity in chloroform-saturated media.
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页码：97 / 102
页数：6
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