ISOLATION OF A FULL-LENGTH CDNA CLONE FOR HUMAN GTP CYCLOHYDROLASE-I TYPE-1 FROM PHEOCHROMOCYTOMA

被引：15

作者：

NOMURA, T

OHTSUKI, M

MATSUI, S

SUMIICHINOSE, C

NOMURA, H

HAGINO, Y

IWASE, K

ICHINOSE, H

FUJITA, K

NAGATSU, T

机构：

[1] FUJITA HLTH UNIV,SCH MED,INST COMPREHENS MED SCI,DIV MOLEC GENET 2 NEUROCHEM,TOYOAKE,AICHI 47011,JAPAN

[2] FUJITA HLTH UNIV,SCH MED,DEPT PHARMACOL,TOYOAKE,AICHI 47011,JAPAN

[3] FUJITA HLTH UNIV,SCH MED,DEPT SURG,TOYOAKE,AICHI 47011,JAPAN

来源：

JOURNAL OF NEURAL TRANSMISSION-GENERAL SECTION | 1995年 / 101卷 / 1-3期

关键词：

GTP CYCLOHYDROLASE I; TETRAHYDROBIOPTERIN; CDNA; MESSENGER-RNA; PHEOCHROMOCYTOMA; (HUMAN);

D O I：

10.1007/BF01271561

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Although the existence of three different cDNA forms of human GTP cyclohydrolase I (GCH I) have been reported (Togari et al., 1992), the full-length sequence of any human GCH I cDNA involving poly (A) tail has not yet been documented. In the present study, we first isolated a full-length cDNA clone encoding human GCH I type 1 from human pheochromocytoma cDNA library. The length of the cDNA insert was 2,921 base pairs including poly (A) tail. RNA blot analysis showed a single mRNA species of 4.0 kb in human pheochromocytoma tissue.

引用

页码：237 / 242

页数：6

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