PURIFICATION OF I-125 LABELED APROTININ BY HYDROPHOBIC INTERACTION CHROMATOGRAPHY

被引：4
|
作者：
RASPI, G
LOMORO, A
SPINETTI, M
机构：
[1] Dipartimento di Chimica e Chimica Industriale, Università di Pisa, Pisa, 56126
来源：
CHROMATOGRAPHIA | 1993年 / 35卷 / 1-2期
关键词：
LIQUID CHROMATOGRAPHY; BOVINE PANCREATIC TRYPSIN INHIBITOR; I-125-LABELED COMPOUNDS;
D O I：
10.1007/BF02278563
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
I-125-aprotinin has been prepared and purified by affinity chromatography in combination with hydrophobic interaction chromatography (HIC). The method allows the preparation of the labelled inhibitor practically carrier-free, in saline solution without organic modifier and the isolated compound retained inhibition activity. Recovery of radioactive material was virtually quantitative, without substantial accumulation of radioactivity on the column. Compared with currently used procedures, the method is more reliable because the labelled polypeptide is collected by following its specific chromatographic signal.
引用
收藏
页码：93 / 96
页数：4
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