PARTIAL-PURIFICATION AND CHARACTERIZATION OF ARYLAMINE N-ACETYLTRANSFERASE IN BOVINE RETINA

被引：7

作者：

GAUDET, SJ

CHADER, GJ

机构：

[1] Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD

来源：

CURRENT EYE RESEARCH | 1992年 / 11卷 / 12期

关键词：

D O I：

10.3109/02713689208999543

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Arylamine N-acetyltransferase (NAT) activity was partially purified and characterized in bovine retina. Upon examining the retinal supernatant for multiple ionic species, only one NAT activity was detected. Based upon its substrate specificity, it is best described as an arylamine NAT. According to size-exclusion HPLC, the molecular mass of the arylamine NAT is approximately 30-kDa. This arylamine NAT acetylates p-aminobenzoic acid thereby demonstrating a monomorphic pattern of acetylation. The NAT activity demonstrated low sensitivity to methotrexate inhibition as indicated by a high IC50 value (480 muM).

引用

页码：1185 / 1192

页数：8

共 50 条

[1] ARYLAMINE N-ACETYLTRANSFERASE (NAT) - PARTIAL-PURIFICATION AND CHARACTERIZATION IN THE BOVINE RETINA
GAUDET, SJ
CHADER, GJ
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 1992, 33 (04) : 1067 - 1067
[2] PURIFICATION AND PARTIAL CHARACTERIZATION OF CHICKEN LIVER ACETYL COENZYME-A - ARYLAMINE N-ACETYLTRANSFERASE
ROUGRAFF, PM
PAXTON, R
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1987, 86 (03): : 601 - 606
[3] Purification and characterization of an arylamine N-acetyltransferase in the nematode Enterobius vermicularis
Chung, JG
MICROBIOS, 1999, 98 (389) : 15 - 25
[4] Purification and characterization of an arylamine N-acetyltransferase from Lactobacillus acidophilus
Chen, GW
Hung, CF
Chang, SH
Lin, JG
Chung, JG
MICROBIOS, 1999, 98 (391) : 159 - 174
[5] Purification and characterization of an arylamine N-acetyltransferase from the bacteria Aeromonas hydrophilia
Chung, JG
CURRENT MICROBIOLOGY, 1998, 37 (01) : 70 - 73
[6] Purification and Characterization of an Arylamine N-Acetyltransferase from the Bacteria Aeromonas hydrophilia
Jing G. Chung
Current Microbiology, 1998, 37 : 70 - 73
[7] Expression, purification, characterization and structure of Pseudomonas aeruginosa arylamine N-acetyltransferase
Westwood, IM
Holton, SJ
Rodrigues-Lima, F
Dupret, JM
Bhakta, S
Noble, MEM
Sim, E
BIOCHEMICAL JOURNAL, 2005, 385 : 605 - 612
[8] ARYLAMINE N-ACETYLTRANSFERASE
SIM, E
HICKMAN, D
CORONEOS, E
KELLY, SL
BIOCHEMICAL SOCIETY TRANSACTIONS, 1992, 20 (02) : 304 - 309
[9] IDENTIFICATION OF ARYLAMINE N-ACETYLTRANSFERASE ACTIVITY IN THE BOVINE LENS
GAUDET, SJ
RAZAVI, P
CAIAFA, GJ
CHADER, GJ
CURRENT EYE RESEARCH, 1995, 14 (10) : 873 - 877
[10] Characterization of arylamine N-acetyltransferase in Enterobacter aerogenes
Tsou, MF
Chung, JG
Wu, LT
Cheng, KS
Hung, CF
MICROBIOS, 1998, 94 (379) : 133 - 143

← 1 2 3 4 5 →