STEPWISE ASSEMBLY OF FUNCTIONALLY ACTIVE-TRANSPORT VESICLES

被引：260

作者：

OSTERMANN, J ^{[1
]}

ORCI, L ^{[1
]}

TANI, K ^{[1
]}

AMHERDT, M ^{[1
]}

RAVAZZOLA, M ^{[1
]}

ELAZAR, Z ^{[1
]}

ROTHMAN, JE ^{[1
]}

机构：

[1] UNIV GENEVA, SCH MED, INST HISTOL & EMBRYOL, DEPT MORPHOL, CH-1211 GENEVA 4, SWITZERLAND

来源：

CELL | 1993年 / 75卷 / 05期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0092-8674(93)90545-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Budding of COP-coated vesicles (the likely carriers of newly synthesized proteins from the endoplasmic reticulum through the Golgi stack) from Golgi cisternae requires ADP-ribosylation factor (ARF), coatomer proteins from the cytosol, GTP, and fatty acyl-coenzyme A (CoA). The assembly of coated buds on the membranes requires coatomer, ARF, and GTP. When palmitoyl-CoA is added, membrane fission occurs at the coated bud, releasing coated vesicles. We show that COP-coated vesicles can be generated stepwise in vitro and isolated in a functionally active form, demonstrating that the minimal set of cytosolic components required for their formation as well as principal steps in their assembly have been identified.

引用

页码：1015 / 1025

页数：11

共 66 条

[1] RECONSTITUTION OF THE TRANSPORT OF PROTEIN BETWEEN SUCCESSIVE COMPARTMENTS OF THE GOLGI MEASURED BY THE COUPLED INCORPORATION OF N-ACETYLGLUCOSAMINE [J].

BALCH, WE ;

DUNPHY, WG ;

BRAELL, WA ;

ROTHMAN, JE .

CELL, 1984, 39 (02) :405-416

[2] SEQUENTIAL INTERMEDIATES IN THE PATHWAY OF INTERCOMPARTMENTAL TRANSPORT IN A CELL-FREE SYSTEM [J].

BALCH, WE ;

GLICK, BS ;

ROTHMAN, JE .

CELL, 1984, 39 (03) :525-536

[3]

BARLOWE C, 1993, J BIOL CHEM, V268, P873

[4] PURIFICATION OF AN N-ETHYLMALEIMIDE-SENSITIVE PROTEIN CATALYZING VESICULAR TRANSPORT [J].

BLOCK, MR ;

GLICK, BS ;

WILCOX, CA ;

WIELAND, FT ;

ROTHMAN, JE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (21) :7852-7856

[5] THE GLYCOPROTEIN THAT IS TRANSPORTED BETWEEN SUCCESSIVE COMPARTMENTS OF THE GOLGI IN A CELL-FREE SYSTEM RESIDES IN STACKS OF CISTERNAE [J].

BRAELL, WA ;

BALCH, WE ;

DOBBERTIN, DC ;

ROTHMAN, JE .

CELL, 1984, 39 (03) :511-524

[6]

CLARY DO, 1990, J BIOL CHEM, V265, P10109

[7] SNAPS, A FAMILY OF NSF ATTACHMENT PROTEINS INVOLVED IN INTRACELLULAR MEMBRANE-FUSION IN ANIMALS AND YEAST [J].

CLARY, DO ;

GRIFF, IC ;

ROTHMAN, JE .

CELL, 1990, 61 (04) :709-721

[8] SEC12P-DEPENDENT MEMBRANE-BINDING OF THE SMALL GTP-BINDING PROTEIN SAR1P PROMOTES FORMATION OF TRANSPORT VESICLES FROM THE ER [J].

DENFERT, C ;

WUESTEHUBE, LJ ;

LILA, T ;

SCHEKMAN, R .

JOURNAL OF CELL BIOLOGY, 1991, 114 (04) :663-670

[9] BREFELDIN-A INHIBITS GOLGI MEMBRANE-CATALYZED EXCHANGE OF GUANINE-NUCLEOTIDE ONTO ARF PROTEIN [J].

DONALDSON, JG ;

FINAZZI, D ;

KLAUSNER, RD .

NATURE, 1992, 360 (6402) :350-352

[10] ADP-RIBOSYLATION FACTOR, A SMALL GTP-BINDING PROTEIN, IS REQUIRED FOR BINDING OF THE COATOMER PROTEIN BETA-COP TO GOLGI MEMBRANES [J].

DONALDSON, JG ;

CASSEL, D ;

KAHN, RA ;

KLAUSNER, RD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (14) :6408-6412

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