A Reactor-Type Biosensor Based on Rhodococcus erythropolis HL PM-1 Cells for Detecting 2,4-Dinitrophenol

被引：0
|
作者：
A. E. Kitova
T. N. Kuvichkina
P. V. Il'yasov
A. Yu. Arinbasarova
A. N. Reshetilov
机构：
[1] Pushchino State University,Skryabin Institute of Biochemistry and Physiology of Microorganisms
[2] Russian Academy of Sciences,undefined
[3] Pushchino,undefined
来源：
Applied Biochemistry and Microbiology | 2002年 / 38卷
关键词：
Alginate; Rhodococcus; Detection Mode; Calcium Alginate; Amperometric Detection;
D O I：
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学科分类号：
摘要：
A model of a reactor-type biosensor based on the Rhodococcus erythropolis HL PM-1 was developed for amperometric detection of 2,4-dinitrophenol (2,4-DNP). The effects of the matrix material (agar and calcium alginate gels, ceramic support, and cellulose powder) on the biosensor signal concentration dependence, detection time, and biosensor stability were studied. In the case of bacterial cells immobilized on cellulose powder, the lower limit of 2,4-DNP detection was 20 μM and the time of single analysis, the biosensor recovery included, was 30–50 min. In the continuous detection mode, the biosensor response was maintained at a stable level without biosensor inactivation for ten days. The biosensor can be used as an element of a complex analytical system for detecting nitroaromatic compounds in samples.
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页码：500 / 505
页数：5
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