Production of DNA-hydrolyzing Antibody BV04-01 Fab Fragment in Methylotrophic Yeast Pichia pastoris

被引：0
|
作者：
A. V. Kozyr
T. V. Bobik
A. N. Ignatova
A. V. Kolesnikov
机构：
[1] Russian Academy of Sciences,Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry
来源：
Molecular Biology | 2004年 / 38卷
关键词：
recombinant antibody production; Fab fragment;
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摘要：
A system was developed for efficient production of recombinant Fab of catalytic DNA-hydrolyzing antibody BV04-01 in methylotrophic yeast Pichia pastoris. To stabilize Fab, the C ends of its chains were modified with dimerizing Jun and Fos, which are known to form a leucine zipper. The yield of functional recombinant Fab was 3 mg per liter culture. The catalytic efficiency of Fab was 1.8 · 106 M–1min–1 as inferred from the relaxation of supercoiled plasmid DNA.
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页码：914 / 920
页数：6
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